Navdeep Kaur, research assistant at NUIG/ Marine Institute, presented a Poster at MaNaPro & ECMNP 2019. The poster aimed to show the management procedures behind the Irish Marine Biodiscovery laboratory, from the collection of samples till the entry of data into the database, passing through the dereplication, biological / chemical screening and prioritization.
A National Marine Biodiscovery Laboratory in Ireland
Navdeep Kaur1,2,*, Laurence K. Jennings1,2, Daniel Rodrigues1,2, Jeffrey Fisher2, Dick FitzGerald3, Alan Dobson4, Dagmar B. Stengel5, Marianela Zanolla5, Grace McCormack6 and Olivier P. Thomas1
The marine environment is a source of an underexplored diversity of macro and microorganisms which are known to produce unique specialized metabolites for communication, defence, and competition. Some marine ecoregions of the oceans have not been inventoried and only sparsely chemically studied like the coasts of the island of Ireland in the North Eastern Atlantic Ocean.
The marine environment is a source of an underexplored diversity of macro and microorganisms which are known to produce unique specialized metabolites for communication, defence, and competition. Some marine ecoregions of the oceans have not been inventoried and only sparsely chemically studied like the coasts of the island of Ireland in the North Eastern Atlantic Ocean.The NMBLI aims to promote marine biodiscovery in Ireland and to bring Ireland to the forefront of global marine research in Europe. Therefore, the NMBLI has developed an Irish marine repository containing freeze-dried biomass, voucher specimens, and fractionated extracts from Irish marine invertebrates. Our repository is organized around a state-of-the-art web-database for storage of data and dereplication of known natural products. The fractionated extracts are chemically screened with UHPLC-HRMS/MS and biologically screened against a broad panel of microbial pathogens and tumour cell lines. These data profiles are uploaded on our web-database and our linked study on the open-access EMBL-EBI’s ‘Metabolights’. This process quickly leads us to the identification of marine organisms for an in-depth chemical investigation. Herein, we present the first results from the dereplication of marine natural products from our marine repository as well as some in-depth chemical investigations that have been performed on promising Irish marine invertebrates.
