A Marine Biodiscovery Laboratory in Ireland

Using both multi-informative molecular network and score-based approaches as prioritization strategies, the Northeastern Atlantic marine terebellid Eupolymnia nebulosa was selected for in-depth chemical investigation. A family of 16 new metabolites named nebulosins was isolated and structurally characterized from extensive analyses of HRMS/MS and NMR spectroscopic data. Nebulosins feature an unprecedented highly substituted thiolane ring leading to up to four contiguous chiral centers. The relative configurations were assigned through a combination of NOESY analysis, spin–spin coupling constant analysis, and NMR chemical shifts measurements, while the absolute configurations were determined by comparison between experimental and theoretical ECD spectra. This family of natural product exhibits promising antioxidant activities in both ORAC and ROS assays.

See the recent publication from Alan Dobson's group at University College Cork as part of the National Marine Biodiscovery project. This paper describes the genome mining and cultivation, as well as a metabolomic analysis, of a Streptomyces sp. isolated from a marine sponge and a terrestrial derived S. albidoflavus to investigate the biosynthesis and production of the known metabolite, Surugamide A. This resulted in the identification factors involved in a differential production of Surugamide A between the marine and terrestrial Streptomyces sp.

https://doi.org/10.3390/microorganisms7100394 See the Abstract below.

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